检疫汉译英200分请手工翻译

Question

干燥或干粉食品：以无菌操作取25g样品，放入装有225ml稀释剂和适量玻璃珠的500ml稀释瓶中。迅速振播，将样品混匀，制成1：10的样品匀液。振播时，幅度为30cm,7s内振播25次，也可用机械振荡器振荡15s代替手摇。
分别加12-15ml平板计数琼脂（已放45+1℃的水浴中恒温）到各平皿内，立即将平皿内的样品液和琼脂培养基充分混合，混合方法是将平皿倾斜和旋转，要防止把混合物溅到平皿壁上和盖上。同时将平板计数琼脂倾入加有1ml稀释剂的另一灭菌平皿做空白对照。将样品液加入平皿后应立即倾注琼脂培养基，每个样品从开始稀释到倾注最后一个平皿所用的时间不得超过20min。
待琼脂凝固后将平皿翻转，立即放进36±1℃的恒温培养箱内培养48±2h,培养箱应保持一定的湿度，经48h培养的琼脂培养基和失重不得超过15%。
通常有三种不同类型的蔓延生长菌落。第一种类型是链状菌落，菌落之间没有明显界线。这些菌落是当琼脂和试验物混合时，一个细菌块被分散所致；第二种类型是在琼脂和平皿底之间形成的水膜样菌落；第三种是在平皿边缘或琼脂表面形成的水膜样菌落。如果所选择的平板出现过量的蔓延菌落生长，以致a.被蔓延菌落盖住的地方，包括由于蔓延菌落造成的抑制生长区面积超过平板面积的50%，或b.由于蔓延菌落造成的抑制生长区面积超过平板面积的25%，这样的平板报告为“蔓延菌落”，不予计数。计数其它平板上的菌落数，将这些数值的算术平均值报告为平板菌落数（下表，样品10）
当有必要技术除以上a.和b.外的蔓延生长菌落时，将三种不同类型的蔓延菌落分别计数。对于第一种类型，如果仅有一条链，将它作为一个菌落计。如果有来源不同的几条链，将每条链作为一个菌落计，不要链上生长的各个菌落分开来数。第二种和第三种类型的蔓延生长形成易于鉴别的菌落，即按一般菌落计数，把技术的蔓延生长菌落数同一般菌落数加在一起，计算平板菌落数。
操作者对同一平板复核自己的计数结果，其差异应在5%之内，而其他人对这一平板重复计数，其差异应在10%之内。否则，应找出原因，加以校正。
适宜稀释度的两个平板的菌落数平均值或两个稀释度的平板菌落数平均值乘以相应稀释倍数计算出每克（毫升）样品中平板菌落数。
用大约175ml的1mol/L氢氧化钠溶液调节pH至7.2，用蒸馏水稀释至1000ml后贮存于冰箱。

稀释液：用蒸馏水稀释1.25ml贮存液至1000ml，分装于合适容器，121℃高压灭菌15min。
本标准主要参考美国食品和药物管理局（FDA）《细菌学分析手册》第6版第4章（1984年）。

Answer 1

Dry or does powder food: Takes the 25g sample by the asepticoperation, puts in is loaded with the 225ml diluent and right amount玻璃珠 500ml 稀释瓶 center. Rapidly inspires broadcasts, mixesuniform the sample, makes 1: 10 samples uniform fluids. Inspires thetime transmission, the scope is in 30cm,7s inspires broadcasts 25times, also may use the mechanical oscillator to vibrate 15s toreplace hand operated.
Separately adds the 12-15ml plate to count the agar-agar (to put in4,5+1 ℃ water baths constant temperature) to each even dish in, willput down in the dish the sample fluid and the agar-agar culture mediumintensive mixing, the mix method will be immediately puts down thedish to incline and to revolve, will have to prevent will splash themixture to the even dish wall on and covers. Meanwhile counts theagar-agar the plate to plunge Canada to have the 1ml diluent anotherantiseptic even dish to do the blank comparison. Joins the even dishafter the sample fluid to be supposed to pour into the agar-agarculture medium immediately, each sample from starts to dilute to poursinto the time which last the even dish uses not to have to surpass20min.
After will wait the agar-agar to coagulate puts down the dish to turnover, will admit immediately 36±1 ℃ in the constant temperatureraise box will raise 48±2h, will raise the box to be supposed tomaintain the certain humidity, will not have to surpass 15% after the48h raise agar-agar culture medium and weightlessness.
Usually has three kind of different types the spread growth colonies.The first kind of type is the chain shape colony, between the colonynot the obvious demarcation line. These colonies are work as whenagar-agar and experiment mix, 细菌块 is dispersed is the result of;The second kind of type is the water film type colony which formsbetween the agar-agar peaceful dish bottom; The third kind is thewater film type colony which forms in the even dish edge or theagar-agar surface. If chooses the plate appears the excessive spreadcolony to grow, so that a. Spreads the place which the colony covers,including because the spread colony creates the suppression growtharea area surpasses the dull area 50%, or b. Because the spread colonycreates the suppression growth area area surpasses the dull area 25%,such plate report is "the spread colony", does not give counts. Countson other plate colony number, these values arithmetic mean valuereports for dull colony number (next table, sample 10)
When has the essential technology except above a. With b. Whenoutside spread growth colony, separately counts three kind ofdifferent types spread colonies. Regarding first kind of type, if onlyhas a chain, takes it a colony idea. If has the origin differentseveral chains, each chain took a colony idea, does not want eachcolony which on the chain grows to separate counts. Second kind of andthe third kind of type spread growth forms the colony which easy todistinguish, namely counts according to the general colony, puts orbring together the technical spread growth colony number identicalcolony number, computation dull colony number.
The operator reexamines to the identical plate own counts the result,its difference should in 5%, but other people count to this platerepetition, its difference should in 10%. Otherwise, should discoverthe reason, performs to adjust.
Is suitable 稀释度 two dull colonies numbers mean value or two稀释度 dull colonies numbers mean value while figure out each gramby the corresponding dilution multiple idea (milliliter) in the samplethe dull colony number.
Adjusts pH with probably the 175ml 1mol/L caustic soda solution to7.2, dilutes with the distilled water after 1000ml stores to therefrigerator.

Diluent: Dilutes the 1.25ml storing fluid with the distilled water to1000ml, loads separately to the appropriate vessel, 121 ℃high-pressured antiseptic 15min.
This standard mainly referred to American food and the medicineadministrative bureau (FDA)"Bacterium Study Analysis Handbook" the 6thedition 4th chapter (in 1984)

不是我抄底！！使我凭感觉写的！！-_-!!怀疑者杀无赦！！

Answer 2

office2007有翻译
Dry or dried noodles food: Takes the 25g sample by the asepsis operation, puts in is loaded with the 225ml diluent and in the right amount beaded glass 500ml dilution bottle.Inspires rapidly broadcasts, mixes uniform the sample, makes 1:10 sample uniform fluid.Inspires the time transmission, the scope is in 30cm,7s inspires broadcasts 25 times, also the available mechanical oscillator vibrates 15s to replace hand operated.
Adds the 12-15ml plate counting agar-agar separately (already puts 45+1℃ in water bath constant temperature) to each even dish in, will put down in the dish the sample fluid and the agar-agar culture medium intensive mixing, the mix method will be immediately puts down the dish incline and revolving, will have to prevent will splash the mixture to the even dish wall on and covers.Meanwhile plunges the plate counting agar-agar Canada to have the 1ml diluent another antiseptic even dish to do the blank comparison.Joins the even dish after the sample fluid to be supposed to pour into the agar-agar culture medium immediately, each sample from starts to dilute to pours into the time which the last even dish uses not to have to surpass 20min. After will wait the agar-agar to coagulate puts down the dish to turn over, will admit immediately 36±1℃ in the constant temperature incubator will raise 48±2h, the incubator will be supposed to maintain certain humidity, will not have to surpass 15% after the 48h raise agar-agar culture medium and weightlessness. Usually has three kind of different type spread growth colonies.The first kind of type is the chaining colony, between the colony does not have the obvious demarcation line.These colonies are work as when agar-agar and experiment mix, a zoogloea is dispersed the result; The second kind of type is the water film type colony which forms between the agar-agar peaceful dish bottom; The third kind is the water film type colony which forms in the even dish edge or the agar-agar surface.If chooses the plate appears the excessive spread colony to grow, so that a. spreads the place which the colony covers, including because the spread colony creates the suppression growth area area surpasses the dull area 50%, or b. because the spread colony creates the suppression growth area area surpasses the dull area 25%, such plate report is “the spread colony”, does not give the counting.Counts on other plate colony number, these value arithmetic mean value report for dull colony number (next table, sample 10) when has in essential technical dividing outside a. and the b. spread growth colony, counts separately three kind of different type spread colonies.Regarding first kind of type, if only has a chain, takes it a colony idea.If has the origin different several chains, each chain took a colony idea, does not want each colony which on the chain grows to separate counts.Second kind of and the third kind of type spread growth forms the colony which easy to distinguish, namely according to the general colony counting, puts together the technical spread growth colony number with the general colony number, computation dull colony number. The operator reexamines own to the identical plate counting result, its difference should in 5%, but other people to this plate repetition counting, its difference should in 10%.Otherwise, should discover the reason, adjusts. The suitable degree of dilution two dull colony number mean value or two degree of dilution dull colony number mean value is multiplied by the corresponding dilution multiple to calculate each gram (milliliter) in the sample the dull colony number. Adjusts pH with probably the 175ml 1mol/L caustic soda solution to 7.2, dilutes with the distilled water after 1000ml stores in the refrigerator. Diluent: Dilutes the 1.25ml storing fluid with the distilled water to 1000ml, loads separately in the appropriate vessel, 121℃ high-pressured antiseptic 15min. This standard mainly referred to American food and medicine administrative bureau (FDA) "Bacteriology Analysis Handbook" the 6th edition 4th chapter (in 1984).

Answer 3

Powder or dry foods: aseptic operation from 25 g sample Add to diluent containing 225ml glass beads and modest dilution of 500ml bottle. Sowing rapid vibration, mixing samples, the samples 1:10 into liquid absorbed. Vibration broadcast, ranges from 30 cm, the vibration 7s broadcast 25 times, can also be used to replace mechanical oscillator oscillating 15s hand.
12-ml and each plate count agar (45 +1 ° C-in thermostat water bath) to the plate, the plate immediately and samples of agar medium mixing, mixing method is to dish tilt and rotation, in order to prevent the mixture splashed on the wall and covered dish. While plate count agar dumped into 1 ml of diluent and a dish to another sterilization blank control. Sample liquid dish by adding immediately after devoted agar, each sample from the beginning diluted into a plate final by the time may not exceed 20 minutes.
After solidification question agar plate overturned, immediately put 36 ± 1 ° C temperature within the incubator of 48 ± 2 hours, the incubator should maintain a certain humidity, the 48h of the agar medium and weightlessness may not exceed 15%.
Usually there are three different types of colony growth spread. The chain is a type of bacteria, no obvious boundary between the colonies. These agar colony when mixing and testing, a bacteria was dispersed by block; The second type is the agar plate, and between the end of the water film-forming bacteria; The third is marginal or agar plate formed on the surface of the water film-like bacteria. If the choice of the spread of flat excessive bacteria growth, resulting in the spread of bacteria a. cover places, including the spread of the bacteria inhibited growth area over the 50% flat area, or b. the spread of the bacteria inhibited growth area over the flat area 25 % in the flat-panel report as "the spread of bacteria," not be counted. Counting the other plate counts, the arithmetic average of these values report to the plate counts (Table sample 10)
Except when necessary technical a. and b. above, the spread of bacteria, it will be three different types of bacteria were spread counting. For the first type, if only one chain, as a colony of it. If there are several different sources chain, each chain as a colony or not all of the chain to several separate colonies. The second and third types of formation easy to identify the spread of the bacteria, as a general colony counting, the spread of technology with the general growth of bacteria colonies together to calculate plate counts.
The operator on the same plate review their counting results, and the differences should be within 5%, while others repeat of this plate count, and the differences should be within 10%. Otherwise, should find reasons to be correct.
For dilution of the two counts of flat or two average dilution of the plate counts multiplied by the average of corresponding dilution calculated per gram (ml) in the sample plate counts.
With about 175ml of 1 mol / L sodium hydroxide solution adjusting the pH to 7.2, diluted with distilled water to 1000ml after stored in the refrigerator.

Dilution: 1.25ml diluted with distilled water to 1000ml of storage, packed in suitable containers at 121 ° C for 15 minutes autoclaves.
The standard main reference to the U.S. Food and Drug Administration (FDA) "bacteriological analysis handbook" version 6 of Chapter 4 (1984).本回答被提问者采纳

Answer 4

没人有这么多时间的，你还是借助工具吧

Dry or does powder food: Takes the 25g sample by the asepticoperation, puts in is loaded with the 225ml diluent and right amount玻璃珠 500ml 稀释瓶 center. Rapidly inspires broadcasts, mixesuniform the sample, makes 1: 10 samples uniform fluids. Inspires thetime transmission, the scope is in 30cm,7s inspires broadcasts 25times, also may use the mechanical oscillator to vibrate 15s toreplace hand operated.
Separately adds the 12-15ml plate to count the agar-agar (to put in4,5+1 ℃ water baths constant temperature) to each even dish in, willput down in the dish the sample fluid and the agar-agar culture mediumintensive mixing, the mix method will be immediately puts down thedish to incline and to revolve, will have to prevent will splash themixture to the even dish wall on and covers. Meanwhile counts theagar-agar the plate to plunge Canada to have the 1ml diluent anotherantiseptic even dish to do the blank comparison. Joins the even dishafter the sample fluid to be supposed to pour into the agar-agarculture medium immediately, each sample from starts to dilute to poursinto the time which last the even dish uses not to have to surpass20min.
After will wait the agar-agar to coagulate puts down the dish to turnover, will admit immediately 36±1 ℃ in the constant temperatureraise box will raise 48±2h, will raise the box to be supposed tomaintain the certain humidity, will not have to surpass 15% after the48h raise agar-agar culture medium and weightlessness.
Usually has three kind of different types the spread growth colonies.The first kind of type is the chain shape colony, between the colonynot the obvious demarcation line. These colonies are work as whenagar-agar and experiment mix, 细菌块 is dispersed is the result of;The second kind of type is the water film type colony which formsbetween the agar-agar peaceful dish bottom; The third kind is thewater film type colony which forms in the even dish edge or theagar-agar surface. If chooses the plate appears the excessive spreadcolony to grow, so that a. Spreads the place which the colony covers,including because the spread colony creates the suppression growtharea area surpasses the dull area 50%, or b. Because the spread colonycreates the suppression growth area area surpasses the dull area 25%,such plate report is "the spread colony", does not give counts. Countson other plate colony number, these values arithmetic mean valuereports for dull colony number (next table, sample 10)
When has the essential technology except above a. With b. Whenoutside spread growth colony, separately counts three kind ofdifferent types spread colonies. Regarding first kind of type, if onlyhas a chain, takes it a colony idea. If has the origin differentseveral chains, each chain took a colony idea, does not want eachcolony which on the chain grows to separate counts. Second kind of andthe third kind of type spread growth forms the colony which easy todistinguish, namely counts according to the general colony, puts orbring together the technical spread growth colony number identicalcolony number, computation dull colony number.
The operator reexamines to the identical plate own counts the result,its difference should in 5%, but other people count to this platerepetition, its difference should in 10%. Otherwise, should discoverthe reason, performs to adjust.
Is suitable 稀释度 two dull colonies numbers mean value or two稀释度 dull colonies numbers mean value while figure out each gramby the corresponding dilution multiple idea (milliliter) in the samplethe dull colony number.
Adjusts pH with probably the 175ml 1mol/L caustic soda solution to7.2, dilutes with the distilled water after 1000ml stores to therefrigerator.

Diluent: Dilutes the 1.25ml storing fluid with the distilled water to1000ml, loads separately to the appropriate vessel, 121 ℃high-pressured antiseptic 15min.
This standard mainly referred to American food and the medicineadministrative bureau (FDA)"Bacterium Study Analysis Handbook" the 6thedition 4th chapter (in 1984

参考资料：http://fanyi.cn.yahoo.com/translate_txt

Answer 5

恩

很好我来了加油