第1个回答 2019-04-14
多肽合成.
萨夫-
23肽合成固相法
芴(芴-甲氧基)
-化学
try.rink
酰胺4
-甲基奔驰hydrylamine
(
mbha
)树脂(
0.55mmol
/克)
,被用来作为支持以获得一个c端酰胺肽.
芴-氨基酸偶联演出具有n
hydroxybenzotriazole
(烷)和dccd
(酋长)
.
氨基酸侧链保护如下:叔(
asp
)
,以及苯甲(谷氨酰胺)
,叔丁(赖氨酸)
.
脱卵裂从树脂实现了混合三氟乙酸,苯酚,水,酚,
1,2
-
ethandithiol
,
和triisopropylsilane
(
88:2.5
:
2.5:2.5:2.5:2.0
,
v
/
v
)的室温条件下反应2h
.
粗肽,然后反复冲洗diethylether
,烘干,真空,
纯化,反相高效液相色谱法对水15毫米deltapak色谱柱(
19
3
30厘米)
.
肽的纯度肽测定分析反相高效液相ultrasphere
c18柱(贝克曼,美国)
460
3
25厘米.
纯化肽酶水解6n
-盐酸,在110℃为22小时,烘干,真空.
残留物溶解于0.02盐酸氮遭受氨基酸分析仪(日立模型,
8500一,日本)
.
肽浓度测定氨基酸分析.
分子量合成肽测定用基质辅助激光解吸电离法(
maldi
)质谱仪.
第2个回答 2019-09-06
每个人都有一个家的人生活在房屋动物有许多不同种不同类型的家庭也有些动物生活在地面，地下洞的土拨鼠他家的生活有两个门，如果敌人成一进门，如果一个人能敌进土拨鼠走出门通过其他一些动物的一些漏洞松鼠在树上筑巢，有些鸟儿在树上生活在洞的鸟在树上筑巢生活中最高的生活乌鸦在树上筑巢高，但老鹰筑巢高山
第3个回答 2019-03-10
亲爱的日记：
我很伤心。我下个月就要回去美国了。我将要和中国还有我的朋友分开。我们下个星期天将会有一个聚会。我很兴奋。每个人都要带礼物。——我会给他一个来自美国的小邮票。——我会给他一个有小提琴音乐的CD。——我会给她一些彩色铅笔。吴一帆喜欢做东西。他将来会当一个科学家。我将会给他一本关于托马斯·爱迪生的书。我很确定他会喜欢的。我不想和我的朋友说再见
第4个回答 2019-07-08
Abstract:
The
so-called
interests
of
consumers,
according
to
the
law
refers
to
the
rights
of
consumers
and
the
right
to
be
protected,
to
bring
consumers
the
benefits
should
be,
the
core
of
which
is
the
rights
of
the
consumers.
And
consumer
rights
-
consumer
spending
for
the
conduct
of
life
should
be
safe
if
equitable
access
to
basic
food,
clothing,
housing
and
medical
care,
such
as
the
right
to
education
in
real
terms,
that
is,
mainly
to
the
basic
human
rights
of
survival.
Along
with
the
socio-economic
development
and
income
level
increases,
the
growing
consumer
demand
for
the
protection
of
transactions
are
in
a
weak
position
in
the
consumer's
legitimate
rights
and
interests,
and
social
and
economic
order,
effective
in
promoting
the
healthy
development
of
market
economy,
the
protection
of
consumer
rights
China
has
been
gaining
attention.
Consumption
is
an
important
element
of
economic
activity
is
the
production
and
distribution
of
the
purpose
of
attribution.
In
order
to
meet
the
consumers
need
to
purchase
personal
life,
the
use
of
the
goods
or
services
received
natural,
economics
consumers
with
the
government,
the
enterprises
to
participate
in
the
parallel
operation
of
the
market
economy
of
the
three
main.
Protecting
the
interests
of
consumers,
it
is
no
longer
as
civil
protection,
as
only
the
interests
of
the
individual
micro-society.
But
the
overall
socio-economic
benefits
from
the
perspective
of
protection
to
ensure
that
the
normalization
of
the
ultimate
consumer
activities
to
promote
economic
development.
第5个回答 2019-10-20
《乱弹琴》
同生今世亦前缘
同尽沧桑一梦间
往事不堪回首论
放生池畔忆前愆
血肉淋漓味足珍
一般苦痛怨难伸
设身处地扪心想
谁愿将刀割自身
《乱弹琴》
还将旧卷续新嗜
莫是东郡频阑素
白首重来谁是伴
我今身亦随网落
鹪鹩但愿巢一枝
鸾停鹄诗意何如
症探西施归入吾
好教情冢定姑宿
《乱弹琴》
纤年溪水太沧浪
幽人独醉月荒凉
泄漏天际又何访
笑偶疯癫皆由它
叼蝉不向耳根来
柳阳到处水西流
仙人白鹤渺烟霞
野日昏昏操暮鸦
《乱弹琴》
字自无意人有意
色自无心人有心
自古情字亦难了
一见穿心天钓命
花若有情还怅望
水应无事莫潺湲
终知此恨销难尽
辜负南华第一篇