高分求英语大神帮忙翻译(英译汉)~医学方向，专业性较强，求只用翻译器弄出的自己都读不通的筒子们绕道…

Question

Assignment of whether protein pairs were orthologous, homologous, paralogous,
or unrelated was assessed manually on an individual basis. Data and evidence
supporting protein similarity are provided in Supplementary Table 1.

EdU/BrdU double labelling.
Parasites labelled with 10 mM EdU and BrdU were
fixed in Methacarn (6:3:1 methanol:chloroform:glacial acetic acid) or processed
for in situ hybridization. After a 45-min 2N HCl treatment, EdU was detected and
parasites were processed for anti-BrdU immunofluorescence (anti-BrdU 1:500,
clone MoBU, Invitrogen). We observed no crossreactivity between this antibody
and EdU.

To quantify the level of BrdU/EdU overlap and measure centre-to-centre distances
between nuclei, three-dimensional confocal stacks from EdU/BrdUlabelled
animals were re-sampled to give isotropic voxels, and subjected to
Gaussian filtering and background-subtraction. Labelled nuclei were segmented
with Imaris (Bitplane Inc.) using parameters empirically determined to minimize
the need for manual corrections; typically, fewer than 5% of the total nuclei
required correction. The three-dimensional coordinates of the nuclei were
exported and analysed with MATLAB. Overlapping EdU- and BrdU-labelled
nuclei were defined as nuclei with centre-to-centre distances ,1 nuclear size
(,4 mm). Statistical analyses were performed in Origin (OriginLab).

Answer 1

Assignment of whether protein pairs were orthologous, homologous, paralogous, or unrelated was assessed manually on an individual basis. Data and evidence supporting protein similarity are provided in Supplementary Table 1.
对蛋白偶对是否为直系同源、异体同源、旁系同源或无关联这项任务分配进行了基于个体的人工评估。支持蛋白质相似性的数据和证据见补充表1。
EdU/BrdU double labeling. Parasites labeled with 10 mM EdU and BrdU were fixed in Methacarn (6:3:1 methanol: chloroform: glacial acetic acid) or processed for in situ hybridization. After a 45-min 2N HCl treatment, EdU was detected and parasites were processed for anti-BrdU immunofluorescence (anti-BrdU 1:500, clone MoBU, Invitrogen). We observed no cross reactivity between this antibody and EdU.
EdU/BrdU双标记。以10mM EdU和BrdU标记的寄生虫固定到麦撒卡恩(methacarn)（6:3:1甲醇：氯仿：冰醋酸）中，或进行现场杂交。经过45分钟的2N赖氨酸处理后，检测到EdU，并对寄生虫进行抗BrdU免疫荧光（抗BrdU 1:500，克隆MoBU，Invitrogen公司）处理。我们观察到这种抗体和EdU之间无交叉反应。
To quantify the level of BrdU/EdU overlap and measure centre-to-centre distances between nuclei, three-dimensional confocal stacks from EdU/BrdU labeled animals were re-sampled to give isotropic voxels, and subjected to Gaussian filtering and background-subtraction. Labeled nuclei were segmented with Imaris (Bitplane Inc.) using parameters empirically determined to minimize the need for manual corrections; typically, fewer than 5% of the total nuclei required correction. The three-dimensional coordinates of the nuclei were exported and analysed with MATLAB. Overlapping EdU- and BrdU-labeled nuclei were defined as nuclei with centre-to-centre distances, 1 nuclear size (, 4 mm). Statistical analyses were performed in Origin (OriginLab).
为了对BrdU/EdU重叠的水平进行量化并测量原子核之间中心到中心的距离，从EdU/BrdU标记的动物中进行了三维共焦架重新采样，以给出各向同性三维像素，并经过高斯滤波和背景减除。标记的细胞核通过经验确定的参数用（Bitplane公司的）伊万里瓷器(imaris) 分割以尽量减少人工校正的需要；通常情况下，只有不到5%的总细胞核需要校正。用MATLAB输出和分析三维坐标细胞核。将EdU和BrdU标记的细胞核的重叠部分即细胞核中心到中心的距离定义为1个细胞核尺寸（,4毫米）。用（Origin实验室的）Origin进行统计分析。

Answer 2

指定的作业是研究一对蛋白质是否直系同源,同系同源,旁系同源或无关的,是手动方式评估基于个别的基础. 补充表1提供了数据和证据支持蛋白质相似性。

乙炔基/溴脲苷(EdU/BrdU)双标记。
寄生元件标记10毫米乙炔基和溴脲苷被固定在石蜡(6:3:1甲醇:氯仿:冰醋酸)或加工为原位杂交。经过45分钟2N盐酸处理后, 检测到乙炔基. 寄生元件处理为溴脲苷的抗体免疫荧光(无性繁殖溴脲苷的抗体MoBu,1:500,培养基)。我们观察到抗体和乙炔基之间没有交叉反应性
为衡量溴脲苷/乙炔基重叠程度和两原子核间距离,三维共焦堆栈从乙炔基/溴脲苷标记动物被重新抽样以提供各向同性体素,并受到高斯滤波和去除背景。以Imaris(Bitplane公司)分割标签核,使用参数经验值以减少手动修正需要;通常,少于总数5%的核需要校正。用矩阵实验室(MATLAB)分析输出的核的三维坐标。重叠的乙炔基和溴脲苷标记核-被定义为与两核间中心距离等于核大小(1.4毫米)。用起源(Origin,(起源实验室))处理统计分析。

Answer 3

分配是否蛋白对同源，同源，同源，或无关的手动进行了评估个人的基础上。表1提供的补充资料和有益的蛋白质相似性。教育/ BrdU双标记。标记10 mm寄生虫埃杜和BrdU经methacarn（6:3:1甲醇：氯仿：冰醋酸）或出原位杂交。45分钟的2N盐酸处理后，检测andparasites教育进行抗BrdU免疫荧光（抗BrdU 1:500，克隆莫不，Invitrogen公司）。我们观察到这antibodyand教育之间无交叉反应。量化的BrdU /教育中心distancesbetween核重叠和测量中心的水平，三维共焦堆栈从教育/ brdulabelledanimals进行重新采样给同性，并受到togaussian滤波和背景减法。标记细胞核segmentedwith imaris（平面Inc.）使用经验确定的函数参数的手动更正需要；通常情况下，只有不到5%的总nucleirequired校正。原子核的wereexported并用MATLAB分析的三维坐标。重叠的教育和BrdU labellednuclei被定义为核的中心到中心的距离，核的大小（1，4毫米）。进行统计分析的起源（originlab）。