都可用，只是do是用于I，you,复数时，does 用于she,he,it,等单数时。具体不懂可追问，我可以给你详细举列。
do 这个词（does是第三人称单数形式），大体上从两方面来讲。
1.作为行为动词，跟其他动词一样，如study, play等。他的意思是“做”等。如：
We usually do our homework in the afternoon.
He does his homework in the evening.
2.作为助动词，也就是帮助主要动词构成否定和疑问等。
We don't like bananas.
Do you like apples?
Does he like English?
He doesn't like swimming.
上面有一句He does his homework in the evening.要是变成否定句就成了这个样子：
He doesn't do his homework in the evening.
这里加上了助动词doesn't，而原来的主动词does变成原形do了。
希望可以帮到你
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