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Question

目的 建立用反相高效液相色谱(RP-HPLC)法同时测定家兔血浆内银杏叶提取物（extract of ginkgo biloba, GBE）中四种黄酮类成分的方法。

方法 三滤醋酸沉淀结合丙酮-乙醚萃取法制备含药血浆。在固定相：Hypersil ODS2 C18色谱柱（5µm，250mm×4.6mm）；流动相：甲醇-0.1%甲酸梯度洗脱；流速：1ml/min；紫外检测波长360nm；柱温：30℃等条件下分析测定。

结果 最低检测浓度0.02µg/ml，方法平均回收率为：芦丁：77.97%，槲皮素：65.53%，山奈酚：69.75%，异鼠李素：68.88%；相对标准差分别为：芦丁：4.43，槲皮素：5.03，山奈酚：4.57，异鼠李素：4.80；标准曲线回归方程为：芦丁：y = 71752x + 714.36，相关系数R2 = 1；槲皮素：y = 160063x - 19861，相关系数R2 = 0.9958；山奈酚：y = 137316x - 1610.8，相关系数R2 = 0.9962；异鼠李素：y = 154985x – 2605，相关系数R2 = 0.996。

结论 该法能很好的分离银杏叶提取物中四种主要黄酮，回收率高，灵敏可靠，重现性好。为GBE的进一步研究提供实验依据。
不要机器翻译

Answer 1

Aims to establish by HPLC (RP-HPLC) method for the determination rabbit plasma Ginkgo biloba extract (extract of ginkgo biloba, GBE) in the four Flavonoids approach.

Acetic acid precipitation method of combining the three filter acetone - Preparation of ether extraction of plasma containing drugs. In stationary phase: Hypersil ODS2 C18 column (5 μ m, 250mm × 4.6mm); mobile phase: methanol -0.1% formic acid gradient elution; velocity: 1 ml / min; UV detection at 360 nm; temperature: 30 ℃ , And other conditions determined.

The detection results of 0.02 μ g / ml, the average recovery methods: Rutin: 77.97%, quercetin: 65.53%, kaempferol: 69.75%, isorhamnetin: 68.88%; relative standard deviation are: Rutin : 4.43, quercetin: 5.03, kaempferol: 4.57, isorhamnetin: 4.80; standard curve regression equation is: Rutin: y = 71752x + 714.36, the correlation coefficient R2 = 1; Quercetin: y = 160063x -- 19,861, the correlation coefficient R2 = 0.9958; kaempferol: y = 137316x - 1610.8, the correlation coefficient R2 = 0.9962; isorhamnetin: y = 154985x - 2605, the correlation coefficient R2 = 0.996.

Conclusion of the Act can be very good from Ginkgo biloba extract in the four major flavonoids, a high recovery rate, sensitive and reliable, reproducible. GBE for further studies provide experimental basis.